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rabbit anti total ulk1  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc rabbit anti total ulk1
    Rabbit Anti Total Ulk1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1156 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/total+ulk1/ULK1+Rabbit+mAb/pm41824459-591-14-17
    Average 96 stars, based on 1156 article reviews
    rabbit anti total ulk1 - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    other:

    Article Title: A clinically relevant decrease in contractile force differentially regulates control of glucocorticoid receptor translocation in mouse skeletal muscle
    Article Snippet: Antibodies against glucocorticoid receptor (Cat. No. 12041), glucocorticoid receptor (Ser211) (Cat. No. 4161), 70 kD ribosomal protein S6 kinase 1 (p70S6K1) Thr389 (Cat. No. 9205), eukaryotic initiation factor 4E binding protein 1 (4EBP1) Ser65 (Cat. No. 9451), microtubule associated protein 1 light chain 3 β (LC3B) (Cat. No. 2775), uncoordinated like kinase 1 (ULK1) Ser757 (Cat. No. 6888), total ULK1 (Cat. No. 8054), and histone H2B (Cat. No. 8135) were obtained from Cell Signaling Technology (Danvers, MA).

    Article Title: Regulation of ubiquitin-proteasome and autophagy pathways after acute LPS and epoxomicin administration in mice
    Article Snippet: Samples were separated by SDS-PAGE for 1 h at a constant intensity of 40 mA and transferred to PVDF membranes at 80 V for 2 h. Membranes were blocked 1 h in 0.1% Tween 20 Tris-buffered saline (TBST) and 5% non-fat dry milk, then incubated overnight at 4°C with one of the following primary antibodies: Regulatory particle triple-A ATPase 1 (RPT1; Enzo, NY, USA), dinitrophenyl (Sigma Aldrich), phospho-Akt Serine(Ser) 473 , total Akt, phospho-factor 4E binding protein 1 (4E-BP1) Threonine(Thr) 37/46 , eukaryotic elongation factor 2 (eEF2), phospho-forkhead box O3a Thr32 (FoxO3a Thr32 ), inhibitor of nuclear factor of kappa B, alpha IκBα, LC3b, LC3a, phospho-p38 Thr180/Tyrosine (Tyr) 182 , phospho-ULK1 Ser757 , total ULK1 (Cell Signaling Technology, Leiden, The Nederlands), p62 (Progen Biotechnik, Heidelberg, Germany) or proteasome 20S α + β (Abcam, Cambridge, UK).

    Article Title: Combined effects of resistance training and calorie restriction on mitochondrial fusion and fission proteins in rat skeletal muscle.
    Article Snippet: Proteins were transferred to polyvinylidene difluoride membranes, and Western blotting was performed using the following primary antibodies: Total OXPHOS Rodent WB Antibody Cocktail (ab110413), fission protein 1 (Fis1, ab96764), dynamin-related protein 1 (Drp1, ab56788), and mitofusin 2 (Mfn2, ab124773) obtained from Abcam (Cambridge, MA); total S6 ribosomal protein (No. 2217), phospho-rpS6 (Ser235/236, No. 2211 and Ser240/244, No. 2215), total ULK1 (No. 8054), phospho-ULK1 (Ser757, No. 14202), LC3B (No. 2775), and phospho-Drp1 (Ser616, No. 3455) obtained from Cell Signaling Technology (Danvers, MA); optic atrophy 1 (Opa1, No. 612606) obtained from BD Transduction Laboratories (Tokyo, Japan); Mfn1 (sc-50330) obtained from Santa Cruz Biotechnology (Santa Cruz, CA); peroxisome proliferator-activated receptor- coactivator-1 (PGC-1 , No. 516557) obtained from Millipore (Billerica, MA); and p62 (PM045) obtained from MBL (Nagoya, Japan).

    Blocking Assay:

    Article Title: Intramuscular injection of mesenchymal stem cells augments basal muscle protein synthesis after bouts of resistance exercise in male mice
    Article Snippet: .. After 5 min blocking with Bullet Blocking One for Western Blotting (13779‐01, Nacalai Tesque, Kyoto, Japan) at room temperature, the membranes were incubated overnight at 4°C with following primary antibodies: GFP (#2956, Cell Signaling Technology), platelet‐derived growth factor receptor alpha (PDGFRα, #3174, Cell Signaling Technology), phosphor‐Akt (Ser473, #9275, Cell Signaling Technology), total‐Akt (#4691, Cell Signaling Technology), phosphor‐p70S6K (Thr389, #9234, Cell Signaling Technology), total‐p70S6K (#2708, Cell Signaling Technology), phosphor‐rpS6 (Ser240/244, #2215, Cell Signaling Technology), total‐rpS6 (#2217, Cell Signaling Technology), phosphor‐4EBP1 (Thr37/46, #9459, Cell Signaling Technology), total‐4EBP1 (#9644, Cell Signaling Technology), ubiquitinated proteins (#3936, Cell Signaling Technology), K48‐linkage‐specific polyubiquitin (#8081, Cell Signaling Technology), Atrogin‐1 (ab168372, Abcam, Cambridge, UK), MuRF‐1 (sc‐398608, Santa Cruz Biotechnology, Dallas, TX, USA), LC3 (#2775, Cell Signaling Technology), p62/SQSTM (PM045, Medical & Biological Laboratory, Nagoya, Japan), phosphor‐ULK1 (Ser757, #14202, Cell Signaling Technology; Ser555, #5869, Cell Signaling Technology), total‐ULK1 (#8054, Cell Signaling Technology), IGFBP3 (10189‐2‐AP, Proteintech, Rosemont, IL, USA), or IGFBP5 (55205‐1‐AP, Proteintech). .. After washing, membranes were incubated with the appropriate secondary antibodies (#7074 or #7076, Cell Signaling Technology) for 1 h at room temperature.

    Western Blot:

    Article Title: Intramuscular injection of mesenchymal stem cells augments basal muscle protein synthesis after bouts of resistance exercise in male mice
    Article Snippet: .. After 5 min blocking with Bullet Blocking One for Western Blotting (13779‐01, Nacalai Tesque, Kyoto, Japan) at room temperature, the membranes were incubated overnight at 4°C with following primary antibodies: GFP (#2956, Cell Signaling Technology), platelet‐derived growth factor receptor alpha (PDGFRα, #3174, Cell Signaling Technology), phosphor‐Akt (Ser473, #9275, Cell Signaling Technology), total‐Akt (#4691, Cell Signaling Technology), phosphor‐p70S6K (Thr389, #9234, Cell Signaling Technology), total‐p70S6K (#2708, Cell Signaling Technology), phosphor‐rpS6 (Ser240/244, #2215, Cell Signaling Technology), total‐rpS6 (#2217, Cell Signaling Technology), phosphor‐4EBP1 (Thr37/46, #9459, Cell Signaling Technology), total‐4EBP1 (#9644, Cell Signaling Technology), ubiquitinated proteins (#3936, Cell Signaling Technology), K48‐linkage‐specific polyubiquitin (#8081, Cell Signaling Technology), Atrogin‐1 (ab168372, Abcam, Cambridge, UK), MuRF‐1 (sc‐398608, Santa Cruz Biotechnology, Dallas, TX, USA), LC3 (#2775, Cell Signaling Technology), p62/SQSTM (PM045, Medical & Biological Laboratory, Nagoya, Japan), phosphor‐ULK1 (Ser757, #14202, Cell Signaling Technology; Ser555, #5869, Cell Signaling Technology), total‐ULK1 (#8054, Cell Signaling Technology), IGFBP3 (10189‐2‐AP, Proteintech, Rosemont, IL, USA), or IGFBP5 (55205‐1‐AP, Proteintech). .. After washing, membranes were incubated with the appropriate secondary antibodies (#7074 or #7076, Cell Signaling Technology) for 1 h at room temperature.

    Incubation:

    Article Title: Intramuscular injection of mesenchymal stem cells augments basal muscle protein synthesis after bouts of resistance exercise in male mice
    Article Snippet: .. After 5 min blocking with Bullet Blocking One for Western Blotting (13779‐01, Nacalai Tesque, Kyoto, Japan) at room temperature, the membranes were incubated overnight at 4°C with following primary antibodies: GFP (#2956, Cell Signaling Technology), platelet‐derived growth factor receptor alpha (PDGFRα, #3174, Cell Signaling Technology), phosphor‐Akt (Ser473, #9275, Cell Signaling Technology), total‐Akt (#4691, Cell Signaling Technology), phosphor‐p70S6K (Thr389, #9234, Cell Signaling Technology), total‐p70S6K (#2708, Cell Signaling Technology), phosphor‐rpS6 (Ser240/244, #2215, Cell Signaling Technology), total‐rpS6 (#2217, Cell Signaling Technology), phosphor‐4EBP1 (Thr37/46, #9459, Cell Signaling Technology), total‐4EBP1 (#9644, Cell Signaling Technology), ubiquitinated proteins (#3936, Cell Signaling Technology), K48‐linkage‐specific polyubiquitin (#8081, Cell Signaling Technology), Atrogin‐1 (ab168372, Abcam, Cambridge, UK), MuRF‐1 (sc‐398608, Santa Cruz Biotechnology, Dallas, TX, USA), LC3 (#2775, Cell Signaling Technology), p62/SQSTM (PM045, Medical & Biological Laboratory, Nagoya, Japan), phosphor‐ULK1 (Ser757, #14202, Cell Signaling Technology; Ser555, #5869, Cell Signaling Technology), total‐ULK1 (#8054, Cell Signaling Technology), IGFBP3 (10189‐2‐AP, Proteintech, Rosemont, IL, USA), or IGFBP5 (55205‐1‐AP, Proteintech). .. After washing, membranes were incubated with the appropriate secondary antibodies (#7074 or #7076, Cell Signaling Technology) for 1 h at room temperature.

    Ubiquitin Proteomics:

    Article Title: Aging Is Associated With Impaired Postprandial Response of Skeletal Muscle Protein Synthesis to High-Intensity Muscle Contraction in Mice.
    Article Snippet: The purpose of this study was to investigate whether aging alters the effect of nutritional status on contraction-induced muscle protein metabolism.. In an overnight fasted or fed states, the right gastrocnemius muscle of young (3 months) and aged (24 months) male C57BL/6J mice was isometrically contracted via percutaneous electrical stimulation.. The left gastrocnemius muscle served as a control.



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    Fig. 5. NGB overexpression regulates autophagy targeting mTOR signaling pathway. CTRL and stably transfected SH-SY5Y-NGB-FLAG cells were lysed in lysis buffer. The lysates were analyzed by western blot to detect P-Ser2448-mTOR or total mTOR protein levels, using anti-P-Ser2448-mTOR pAb or anti-total mTOR pAb or <t>P-Ser757-ULK1</t> or total ULK1 using anti-P- Ser757-ULK1 pAb. Loading control was evaluated using mouse anti-Beta-Actin mAb. Results represent the mean ± SD from three independent experiments (n = 3). *** p < 0.001. Uncropped blot images are shown in Supplementary Information file.
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    Fig. 5. NGB overexpression regulates autophagy targeting mTOR signaling pathway. CTRL and stably transfected SH-SY5Y-NGB-FLAG cells were lysed in lysis buffer. The lysates were analyzed by western blot to detect P-Ser2448-mTOR or total mTOR protein levels, using anti-P-Ser2448-mTOR pAb or anti-total mTOR pAb or <t>P-Ser757-ULK1</t> or total ULK1 using anti-P- Ser757-ULK1 pAb. Loading control was evaluated using mouse anti-Beta-Actin mAb. Results represent the mean ± SD from three independent experiments (n = 3). *** p < 0.001. Uncropped blot images are shown in Supplementary Information file.
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    Image Search Results


    Fig. 5. NGB overexpression regulates autophagy targeting mTOR signaling pathway. CTRL and stably transfected SH-SY5Y-NGB-FLAG cells were lysed in lysis buffer. The lysates were analyzed by western blot to detect P-Ser2448-mTOR or total mTOR protein levels, using anti-P-Ser2448-mTOR pAb or anti-total mTOR pAb or P-Ser757-ULK1 or total ULK1 using anti-P- Ser757-ULK1 pAb. Loading control was evaluated using mouse anti-Beta-Actin mAb. Results represent the mean ± SD from three independent experiments (n = 3). *** p < 0.001. Uncropped blot images are shown in Supplementary Information file.

    Journal: Scientific reports

    Article Title: Neuroglobin regulates autophagy through mTORC1/RAPTOR/ULK-1 pathway in human neuroblastoma cells.

    doi: 10.1038/s41598-025-91701-w

    Figure Lengend Snippet: Fig. 5. NGB overexpression regulates autophagy targeting mTOR signaling pathway. CTRL and stably transfected SH-SY5Y-NGB-FLAG cells were lysed in lysis buffer. The lysates were analyzed by western blot to detect P-Ser2448-mTOR or total mTOR protein levels, using anti-P-Ser2448-mTOR pAb or anti-total mTOR pAb or P-Ser757-ULK1 or total ULK1 using anti-P- Ser757-ULK1 pAb. Loading control was evaluated using mouse anti-Beta-Actin mAb. Results represent the mean ± SD from three independent experiments (n = 3). *** p < 0.001. Uncropped blot images are shown in Supplementary Information file.

    Article Snippet: Membranes were blocked with 5% w/v non-fat dried milk (sc-2325, Santa Cruz Biotechnology, Dallas, Texas, USA) in Tris buffer saline (1706435, Bio-Rad Laboratories) (TBS), containing 0.05% v/v Tween 20 (1706531, Bio-Rad Laboratories) (TBST) and probed with rabbit anti-LC3 pAb (NB100-2331, Novus Biologicals, Centennial, Colorado, USA), rabbit anti-SQSTM1 mAb (Cell Signaling Technology, Danvers, Massachusetts, USA), rabbit anti-P-Ser757-ULK1 pAb (6888, Cell Signaling Technology), rabbit anti-P-Ser2448-mTOR pAb (2971, Cell Signaling Technology), rabbit total ULK1 mAb (8054, Cell Signaling Technology) or rabbit anti-total mTOR mAb (2983, Cell Signaling Technology).

    Techniques: Over Expression, Stable Transfection, Transfection, Lysis, Western Blot, Control